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Reconstitution Calculator

Peptide Reconstitution
Calculator

Enter your peptide vial size and target concentration to instantly calculate the exact volume of bacteriostatic water to add and the resulting concentration in mg/mL and mcg/mL.

BAC water volume 12 compound presets

Quick Presets

Enter Your Values

The amount of lyophilized peptide in the vial (printed on label)

Common values: 200, 500, 1000, 2000 mcg/mL

For laboratory research use only. This tool converts amounts and volumes; it does not give dosing or use instructions. Check the arithmetic before you mix.

BAC Water to Add

10.00 mL

of bacteriostatic water → 500 mcg/mL (0.500 mg/mL)

Reconstitution Summary

Peptide amount5 mg (5000 mcg total)
BAC water to add10.00 mL
Final concentration500 mcg/mL (0.500 mg/mL)
Label the vial500 mcg/mL, date mixed, diluent

How Peptide Reconstitution Works

Research peptides are supplied as lyophilized (freeze-dried) powder in sealed vials. Before use, the powder must be dissolved in bacteriostatic water (BAC water) — a process called reconstitution. The volume you add sets the concentration of the solution, which is what goes on the label.

1

Choose your concentration

Pick the concentration you want in the vial (for example 1 mg/mL or 2.5 mg/mL) and check the vial can hold the volume. Less water = higher concentration.

2

Add BAC water slowly

Measure the calculated BAC water volume and let it run slowly down the inside wall of the vial. Do not aim the stream at the powder; turbulence can damage the peptide.

3

Swirl, don't shake

Gently swirl the vial until the powder is fully dissolved. Do not shake — agitation can break peptide bonds. Store at 2–8°C, protected from light.

The Formula

BAC water (mL) = Peptide (mg) × 1,000 ÷ Target concentration (mcg/mL)

Example: 5 mg peptide at 500 mcg/mL → 5 × 1,000 ÷ 500 = 10 mL BAC water

Frequently Asked Questions

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