GPR54 Agonism & Neuroendocrine Axis Regulation
Kisspeptin-10 — GPR54 Receptor Agonist
Kisspeptin-10 (KLOW) is the biologically active C-terminal decapeptide fragment of kisspeptin, a neuropeptide encoded by the KISS1 gene that serves as the master regulator of the hypothalamic-pituitary-gonadal (HPG) axis. Discovered in 2001 as the endogenous ligand for the orphan receptor GPR54 (now designated KISS1R), kisspeptin has since been recognized as the critical upstream signal that gates the onset of puberty and regulates reproductive function throughout life.
Kisspeptin-10 retains full biological activity at GPR54 and is the most commonly used research form due to its smaller size and ease of synthesis compared to the full 54-amino-acid kisspeptin-54 precursor. Its role extends beyond reproduction: kisspeptin neurons integrate metabolic signals (leptin, insulin, glucose) and relay this information to GnRH neurons, making kisspeptin a key node linking nutritional status to reproductive and metabolic function.
Kisspeptin-10 is a 10-amino-acid peptide with the sequence Tyr-Asn-Trp-Asn-Ser-Phe-Gly-Leu-Arg-Phe-NH2. The C-terminal amide group and the RF-amide motif (Arg-Phe-NH2) at the C-terminus are critical for GPR54 binding. The molecular weight is 1,302.4 Daltons.
Kisspeptin-10 corresponds to the C-terminal 10 residues of kisspeptin-54 and retains full GPR54 agonist activity. The RF-amide motif is a conserved pharmacophore shared by a family of neuropeptides that includes neuropeptide FF and neuropeptide AF.
Kisspeptin-10 acts as an agonist at GPR54 (KISS1R), a Gαq/11-coupled receptor expressed on GnRH neurons in the hypothalamus, pituitary gonadotrophs, and peripheral tissues. GPR54 activation stimulates phospholipase C (PLC), generating IP3 and DAG, which mobilize intracellular calcium and activate PKC. This signaling cascade depolarizes GnRH neurons, triggering GnRH release into the hypothalamic-pituitary portal circulation.
GnRH then stimulates pituitary gonadotrophs to release LH and FSH, which act on the gonads to stimulate sex steroid production and gametogenesis. This complete HPG axis activation makes kisspeptin-10 a powerful research tool for studying reproductive endocrinology, puberty, and the neuroendocrine regulation of fertility.
KLOW is a proprietary research blend combining GLP-1 (the hormone behind Ozempic), GLP-2 T (the FDA-approved gut repair peptide), and GLP-3 R (Retatrutide, the triple-receptor agonist with 24% weight loss data). The idea is to combine the metabolic and appetite-regulating effects of all three compounds into a single research formulation.
Each component targets a different aspect of metabolic health: GLP-1 handles glucose regulation and appetite suppression, GLP-2 T supports gut integrity and nutrient absorption, and GLP-3 R adds the triple-receptor metabolic boost including glucagon-mediated fat burning. Together, the blend theoretically covers the full spectrum of GLP-axis biology — from blood sugar and appetite to gut health and energy expenditure.
The GLP axis is currently the most active area in metabolic drug development. A research blend that combines three complementary GLP-axis peptides allows researchers to study the combined effects of this system in a way that individual compounds don't permit. It's a sophisticated tool for researchers working at the intersection of obesity, metabolic syndrome, and gut biology.
KLOW is a high-complexity research blend for advanced metabolic research. As with all blends, direct studies on this specific combination are limited; the rationale is based on the complementary mechanisms of the three individual components. All three are research-only compounds. This is one of the most scientifically ambitious formulations in the Purgo Labs catalog.
Activates Gαq/11 → PLC → IP3/DAG → Ca²⁺ mobilization cascade, depolarizing GnRH neurons and triggering GnRH release.
GnRH release stimulates pituitary LH and FSH secretion, activating gonadal steroidogenesis and gametogenesis.
Kisspeptin neurons receive leptin, insulin, and glucose signals, relaying metabolic status to the reproductive axis.
c-Src/FAK pathway modulation by kisspeptin-10 inhibits tumor cell migration and angiogenesis in cancer models.
Metabolic Research
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| Peptide Class | Kisspeptin fragment (10 amino acids, RF-amide) |
| Molecular Weight | 1,302.4 Da |
| Receptor Target | GPR54 / KISS1R (Gαq/11-coupled) |
| Key Pharmacophore | C-terminal RF-amide motif (Arg-Phe-NH2) |
| Available Sizes | 10mg vials |
| Form | Lyophilized powder |
| Purity | ≥99% (third-party tested) |
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Purchase KLOW at Purgo LabsMedical Disclaimer: All content on this site is for educational and research purposes only. Research peptides are not FDA-approved for human use. Always consult a qualified healthcare professional before considering any peptide or supplement protocol. Nothing on this site constitutes medical advice, diagnosis, or treatment.
